This month we look at a simple and structured approach to developing a reversed phase Solid Phase Extraction (SPE) method. We’ve provided an interactive tool to allow you to evaluate different sets of conditions and see what effect they have on the resulting chromatogram.more »
1) Before switching on gases, set the temperature of the detector and allow to equilibrate for at least 30mins after reaching operating temperature. This ensures all surfaces are hot and dry, creating the right.more »
1) Mobile phases on correct pump line2) Sufficient mobile phase present to complete analysis of full run3) Each mobile phase line purged with at least 30mlmore »
1) Not Taking Enough Care at the Inlet2) Turning the oven on before the carrier gas3) Not programming in column dimensionsmore »
We take a look at the important topic of buffer choice for HPLC separations, how to the choose the correct buffer type and concentration as well as how to avoid variability in retention and selectivity. more »