1) Measuring the pH of the mobile phase after the organic has been added
2) Not using a buffer
3) Not using the buffer in its correct pH range
4) Adding buffer to organic
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Troubleshooting retention time variability in HPLC
Everyone doing HPLC analysis has experienced drifting or unstable analyte retention times at some point – usually just as you’re about to head home! Some quick tips on how to avoid the problems and improve method robustness more »
10 points to understand Electrospray Ionisation
It’s often difficult in the busy laboratory to find time for leaerning and development. CHROMacademy delivers key knowledge, quickly – and to prove it here is our 10 points to Understanding Electrospray Ionisation! more »
Gradient HPLC Quiz
The gradient elution mode in HPLC can be very useful but how much do you really know about the mechanisms and manipulating gradients to effect a separation? Find out with our quick 10 question quiz! Take the quiz »
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An SPE method follows 5 steps:
1) Sample pretreatment - e.g. pH adjustment, protein precipitation, filtration
2) Column conditioning - the column is made ready for the sample being loaded. more »
