Understanding GC Methods: Part I Sample Introduction Variables

The Essential Guide from LCGC’s CHROMacademy presents the first in a series of webcasts aimed at developing the skills and understanding of GC users. In this session, Dr. John Hinshaw (Senior Scientist, BPL Global Ltd) and Tony Taylor (Technical Director, Crawford Scientific) take an in-depth look at principles of GC sample introduction and the associated variables which need to be defined in GC methods. We describe the principles of sample injection and describe in detail how method variables are set and optimized using real life ‘good’ and ‘bad’ method examples.

View October Tutorial & on Demand Webcast »

• Understand the working principles of the Split / Splitless Injector
• Appreciate the major and minor variables which control sample injection
• Recognize when a method is deficient in terms of the amount of information given and make informed choices about the parameters which are not stipulated
• Appreciate the importance of proper inlet function and make informed choices in terms of inlet liners
• Understand how to set and optimize sample injection variables based on sample (analyte) type and intended analytical outcomes

CHROMacademy Content

• Sample Introduction
• Sampling Techniques
• GC Temperature Programming

• A Split Decision
• Practical Guidelines in the Care and Maintenance of Capillary GC Columns
• The LCGC Blog: Chromatography Technical Tips - Five Ways to Improve Your Split/Splitless Injection Technique
• Measuring Gas Flow for Gas Chromatography

CHROMacademy Webcasts / Essential Guides

• Split/Splitless Injection
• GC Troubleshooting Gas Supply and Inlet Issues
• GC Troubleshooting Masterclass
• Translating GC and GCMS Methods