Developing Better HPLC (MS) Methods

Where do you start when you want to develop a better HPLC method? Whether you work in a regulated environment or not, setting specifications for your HPLC protocols is a good practice. In this Essential Guide we will set out guidelines for chromatographic parameters such as retention, resolution, and efficiency that can be used to improve your HPLC methods prior to method validation. We will explain how mobile phase design and instrument parameters can be used to achieve improvements in method robustness and what to look for when a method is failing. Practical tips for sample and eluent preparation, and the correct detector settings to use will also be discussed.

View September Tutorial & on Demand Webcast »

• Understand what specifications should be in place so that HPLC methods are robust and reproducible
• Learn good practice for sample preparation and injection
• Understand the importance of using the correct detector settings for your analysis
• Appreciate the variables in LC(MS) methods which effect robustness
• Develop knowledge of how to alter retention and selectivity of analytes
• Gain practical tips to improve your method development process

CHROMacademy Content

• Reversed Phase (Partition) Chromatography
• Column Chemistry with Webcast!
• Fundamental LCMS Introduction

• How Reversed Phase LC Works
• A Three-Pronged Approach for Rapid HPLC Method Development
• Buffer Considerations for LC and LC-MS

CHROMacademy Webcasts / Essential Guides

• Developing Better Methods for Reversed Phase HPLC (Part 2)
• Optimizing LC-MS and LC-MS/MS
• LCMS Methods Parameters – Critical Evaluation
• Understanding HPLC Column Characterization