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Featured Application Note:
On-Site Quality Control of Beer
Sonja Schneider
This application note shows the analysis of isohumulones in different beer samples using the Agilent 1220 Infinity Mobile LC solution. Read More |
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Automated Solid Phase Extraction and Concentration for the Determination of Nitrosamines in Drinking Water
Phillip Bassignani, FMS, Inc
Nitrosamines are a group of chemical compounds regulated because of their carcinogenic characteristics. Nitrosamines have many sources including widespread usage in the manufacturing of rubber products, as well as cosmetics. They have also been demonstrated to form from reactions between food preservatives and amines. In drinking water, established limits are low (<1 ng/L), requiring detection by a mass spectrometer in the chemical ionization mode Read More |
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Analysis of Pesticide Residue in Spinach
Tyler Trent, Teledyne Tekmar
This study was conducted to evaluate the performance and versatility of the AutoMate-Q40. an automated QuEChERS solution. A liquid chromatography-tandem mass spectrometry (LC–MS–MS) method was used to determine 14 pesticides from spinach using the AutoMate-Q40. Quantification was based on matrix-matched calibration curves with the use of internal standard to ensure method accuracy. QC samples were evaluated at levels of 20-, 40-, and 100-ng/g to ensure precision and accuracy of the AutoMate-Q40. Read More |
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Determination of Tetracycline Antibiotics in Milk Using a Simple Strong Cation-Exchange SPE Cleanup Procedure and LC–MS–MS Analysis
Brian Kinsella, UCT Inc.
Tetracyclines (TC) are broad spectrum antibiotics widely used in food animals. To ensure food safety and minimize antibiotic drug exposure to consumers, tetracyclines are included in chemical residue surveillance plans. This study demonstrates a simple, efficient procedure for the extraction and quantification of TC antibiotics in milk. The method allows for the rapid analysis of TCs in milk while achieving good accuracy, precision, and sensitivity. Read More |
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GPC–SEC Analysis of Hydroxyethyl Cellulose Used in Pharmaceutical Products
Stephen Luke, Agilent Technologies
Suspension and viscosity-increasing polymer excipients, for example hydroxyethyl cellulose, can be used to uniformly disperse ingredients and maintain suspension so actives do not precipitate or settle under gravity, particularly valuable for liquid formulations during or after manufacture. Read More |
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UHPLC with Light Scattering
Wyatt Technology
The very first multi-angle light scattering (MALS) detector for UHPLC produces SEC–MALS results that rival the best GPC results – with a fraction of the solvent used. Read More |
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Hydrogen Performance for Carrier Gas
Ed Connor, Peak Scientific
Many labs are facing supply difficulties of helium, one of the most commonly used carrier gases and as a consequence are facing rapid price increases. Many chromatography labs are therefore looking for alternative carrier gases and show how hydrogen can be used as an alternative to helium, as well as improving the separation of analytes in a complex mixture of compounds whilst improving the throughput of sample.
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GC–MS–MS Analysis of Pesticides in Baby Foods
Shimadzu
GC–MS has been used extensively to identify and quantify trace-level pesticides in food matrices. This note presents results for analysis of 36 pesticides from various chemical classes in a QuEChERS extract of baby food. Results were evaluated for calibration linearity, analytical precision, and accuracy; selectivity as a function of variable mass spectral resolution settings on the two sets of quadrupoles is also discussed. Read More |
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Evaluation of Matrix Effects for Dilute-and-Shoot LC–MS–MS Analysis of Carbendazim in Orange Juice and Wine
Helen (Qingyu) Sun, Zicheng Yang, and Kefei Wang, Bruker Daltonics
A quantitative multiple reaction monitoring (MRM) method for carbendazim analysis in orange juice and wine matrices was developed for the Bruker EVOQ Elite LC–MS–MS system with a calibration range from 0.005 up to 50 ppb. Matrix effects were evaluated by comparing matrix effect % against dilution factors. Results indicate that orange juice and wine matrices exhibit different matrix effects and provide the scientific reference to determine appropriate dilution factors. Read More |
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Amino Acid Analysis of Cell Culture Media
Maria Ofitserova, PhD, Pickering Laboratories
Monitoring and adjusting amino acid composition of cell culture media is an essential part of optimizing the manufacturing process to ensure high quality and optimum yield of the final product. Read More |
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Analysis of Monoamine Neurotransmitters by Polymer-Based Ion Chromatography and Mass Spectrometry
Melissa Turcotte and Junji Sasuga, Shodex/ Showa Denko America, Inc.
Shodex YS-50, a polymer based ion chromatography column for cation analysis with nonsuppressor methods, has demonstrated the separation of monoamine neurotransmitters by single ion mode MS. Read More |
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Improving Recovery and Resolution of mAb Aggregates Using Mobile Phase Additives in SEC
Tosoh Bioscience
The possibilities of mobile phase enhancement are often overlooked in SEC, since it is thought to leave less room for improvement when compared to other chromatographic conditions such as particle size, packing quality, and column length. Once certain mobile phase parameters have been set, such as pH, stationary phase, and ionic strength, significant improvements can, in fact, be made to mAb aggregate separation by mobile phase additives. This application note will show how SEC analysis of mAb aggregate samples using a TSKgel® UltraSW Aggregate column are improved by amino acid additives in the mobile phase. Read More |
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Scientific Search in the Science-Based Organization
Waters Corporation
Access to information is central to the success of science-driven organizations. These organizations need an Informatics solution that provides timely, regular, accurate, and useful search results to deliver benefits across multiple layers of the organization. A new generation of searching solution is required for science-driven organizations: one that extends across internal information repositories, handles science objects, and can index unstructured data. Read More |
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21 CFR Part 11 Readiness of Empower 3
Waters Corporation
Meeting 21 CFR Part 11 compliance remains challenging; however, e-record regulations will eventually be viewed as a significant driver to move companies from a paper-records environment to a more efficient electronic-records environment. Although it is understood that merely purchasing a chromatography data software package that incorporates Part 11 technical controls does not make one fully compliant, technical controls should be inherent in any system used in a regulated environment. A suite of technical controls for 21 CFR Part 11 compliance are built into Empower 3 Software to simplify administration and allow laboratories to meet the U.S. and European regulations. Read More |
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Scientific Search in the Science-Based Organization
Waters Corporation
Access to information is central to the success of science-driven organizations. These organizations need an Informatics solution that provides timely, regular, accurate, and useful search results to deliver benefits across multiple layers of the organization. A new generation of searching solution is required for science-driven organizations: one that extends across internal information repositories, handles science objects, and can index unstructured data. Read More |
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21 CFR Part 11 Readiness of Empower 3
Waters Corporation
Meeting 21 CFR Part 11 compliance remains challenging; however, e-record regulations will eventually be viewed as a significant driver to move companies from a paper-records environment to a more efficient electronic-records environment. Although it is understood that merely purchasing a chromatography data software package that incorporates Part 11 technical controls does not make one fully compliant, technical controls should be inherent in any system used in a regulated environment. A suite of technical controls for 21 CFR Part 11 compliance are built into Empower 3 Software to simplify administration and allow laboratories to meet the U.S. and European regulations. Read More |
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