How to choose an HPLC Column |
Some great general hints and tips for HPLC column selection.
Read the literature
It is very rare that a separation has never been performed before and so checking the literature, refereed journals...
Run a generic method
So you have read the literature and still have not found a separation... More »
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5 ways to improve your Split / Splitless Injection technique |
1. Understand the process...
Split/splitless injectors typically vaporize a sample dissolved in a
suitable organic solvent under increased temperature. The sample
vapors are entrained into the carrier gas flow inside a ‘liner’ or
‘sleeve’ within the inlet and from there pass into the column or out of
the inlet via the ‘split’ line/valve. More »
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Does Mass Spectrometry Need Chromatography? |
I am amazed by how often I hear someone say that mass spectrometry
doesn’t need chromatography. I have mentioned before that I feel I need a
reversible t-shirt that says, “I am a chromatographer” on one side and
“I am a mass spectrometrist” on the other. More »
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Don’t let your HPLC column become just another statistic of abuse! Read
these few short questions on HPLC column myths and be kind to your
columns. They’ll appreciate it and work better for you. More »
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HPLC Troubleshooting Guide to Split Peaks
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Solving HPLC Peak Splitting Problems using the CHROMacademy HPLC
Troubleshooter. Split peaks, increased pressure, erratic baselines, loss
of resolution, leaking HPLC pumps…..and to top it all off an ever
mounting pile of samples that needed run yesterday! Keep calm and
use our interactive HPLC Troubleshooter to get everything back on track.
Start Troubleshooting »
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GC Method Development Course
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If you have ever needed to tweak a temperature program, optimize sample injection conditions, obtain a better limit of detection, improve resolution, increase sample throughput or transfer a method then this course is for you. CHROMacademy LIVE on the Web - Starts September 2014 »
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Celebrating 5 Years of Online Training
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CHROMacademy - going from strength to strength!
This Year Our 27,000 members have viewed over 1.5 million pages, taken 24,000+ assessments and accessed 9,500 hours of webcast/video. www.CHROMacademy.com |
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