Some great general hints and tips for HPLC column selection. Read the literature: It is very rare that a separation has never been performed before and so checking the literature, refereed journals... Run a generic method: So you have read the literature and still have not found a separation...
1) Measuring the pH of the mobile phase after the organic has been added
2) Not using a buffer
3) Not using the buffer in its correct pH range
4) Adding buffer to organic
If there’s one mantra that’s essential for an LC (or GC)-MS operator it’s “contamination, contamination, contamination”. Keep contamination to a minimum and you’ll enjoy a reliable instrument; don’t take the necessary precautions and instead you’ll enjoy reaching for the vent switch and purchasing lots of spares. More »
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