|
|
|
 |
Separation of Apo-Transferrin and Bovine Serum Albumin (BSA) Proteins
Janusz Zukowski, Diamond Analytics
The unique surface chemistry of the FLARE diamond core-shell column combines ionic and hydrophobic separation mechanisms to effectively retain a variety of chemical species in a single run. Read More |
 |
Investigation of Synthetic Compounds in Drug Discovery by Integrated HPLC and LC–MS
Shimadzu
This paper introduces an analysis of eight pharmaceutical substances in a workflow that is typically used for pharmaceutical synthesis confirmation analysis. It describes an example in which measurement was conducted for a range of compounds with properties spanning acidic to basic, and high to low polarity. Read More |
|
A New HILIC Column for Saccharide Analysis
Melissa Turcotte and Naoya Nakajima, Shodex/Showa Denko America, Inc.
Shodex introduces a new HILIC column for the analysis of sugars. The new column, NGP-50, has polymer packing material with chemically stable tertiary amino functional groups. Read More |
|
2D-LC Separation of Cationic, Anionic, and Hydrotropic Surfactants
Justin Steve, Tosoh Bioscience
Because surfactants can be polar, non-polar, or amphoteric, the structural diversity of the surfactants and complexity of the sample matrix can make their separation and identification by HPLC challenging. Historically, this has been achieved using reversed-phase chromatography supplemented with ion-exchange chromatography to characterize the surfactant profile. This application note illustrates the effectiveness of a reversed-phase high throughput column, TSKgel® ODS-140HTP, in series with a TSKgel NH2-100 HILIC column, for the separation of polar and nonpolar surfactants in a single injection using 2D–LC. Read More |
|
|
|
|
| |
 |
Automated SPE and Cleanup for PCBs in Human Serum
Philip Bassignani, FMS, Inc.
The extraction of PCBs from human serum using solid-phase extraction (SPE) coupled with multi-column silica gel clean-up procedures is a well documented procedure. It offers a rapid sample prep protocol, while utilizing minimal organic solvent. Using this template, a rapid screening method has been established for 40 PCBs and their labeled analogs using FMS automated solid-phase extraction and clean-up systems.
Read More |
|
|
Determination of Deoxynivalenol in Shredded Wheat Cereal
Toni Hofhine, Horizon Technology, Inc., Elizabeth K. Krantz, Dr. Pamela Doolittle, and Dr. Cheri A. Barta, University of Wisconsin-Madison
Deoxynivalenol is a common mycotoxin found in agricultural grain crops and final consumer processed products. Most impacted are wheat, barley, and corn. Deoxynivalenol, also known as vomitoxin, has the ability to withstand high processing temperatures, creating the need for rapid and accurate determination methodology.
Read More |
|
|
Free Opiates and Glucuronides in Urine Extracted by Clean Screen® DAU and Analyzed by LC–MS–MS
Jody Searfoss, UCT, Inc
Opiates act as narcotic analgesics that depress the central nervous system. Positively-screened specimens usually contain opiate glucuronide conjugates in addition to free opiates. Typical confirmation includes direct opiate analysis without a hydrolysis protocol being conducted. This allows for the determination of parent opiates, metabolites, and glucuronide metabolites. This method uses mixed-mode solid-phase extraction and LC–MS–MS for the extraction of both free opiates and glucuronides in urine.
Read More |
|
|
| |
 |
Validation of EPA Method 8260C on Thermo Trace 1300 with New Helium Saver Inlet
Jacob Rebholz, Teledyne Tekmar
As helium continues to become more expensive, many labs are searching for more cost-effective carrier gas options. This study demonstrates the effectiveness of the Teledyne Tekmar's Atomx automated sample prep system's nitrogen purge feature in conjunction with Thermo Scientific's Trace 1300 series Helium Saver inlet at producing quality data while also reducing helium consumption. Calibration and method detection limit (MDL) samples were prepared, analyzed, and quantitated according to US EPA Method 8260C. Read More |
|
|
|
|
| |
 |
SEC-MALS of Transient Proteins
M. Burman and O. Schon
While AUC may be the gold standard for the determination of self-association in solution, scientists are looking for alternatives due to AUC’s high costs, low throughput, and expertise requirements. Here, SEC-MALS has shown excellent agreement with AUC results and can be used as a cheaper, easier, and quicker alternative.
Read More |
|
|
Characterization of Protein-Protein Interactions by Size Exclusion Chromatography
Bertrand Raynal, Patrick England, Nadia Benaroudj, and Alex Chenal, Institut Pasteur, Paris, France
In this application note, triple detection size-exclusion chromatography (SEC–TD) is used to understand the ability of proteins to form ordered, structured complexes. The controlling mechanism behind these protein complexes is fundamental to our knowledge of protein signaling pathways and the physiological responses they control.
Read More |
|
|
| |
 |
Rapid Screening of Drugs of Abuse Using Paper Spray MS
Joseph H. Kennedy and Justin M. Wiseman, Prosolia, Inc.
Paper spray mass spectrometry (MS) allows for the direct analysis of pharmaceuticals, drugs of abuse, and other small molecules from blood, urine, and other biofluids. Described herein is the application of a Velox 360 paper spray autosampler and ion source for the direct analysis of drugs of abuse in urine samples.
Read More |
|
|
|
