The CHROMacademy Essential Guide Tutorial - April 2015
Techniques Employed in Biopharmaceutical Analysis - What, Why, and How
Part II Ion Exchange and SEC
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Biomolecules are complex by nature making their characterization challenging. Often numerous chromatographic techniques will be employed to allow the full characterization of a biomolecule; this may be in its intact state or fragmented state (i.e. glycan. peptide, amino acid analysis). Part I of our two part event discussing the techniques used in the analysis of biopharmaceuticals focused on reversed phase and HILIC. Part two focusses on ion exchange (IEX), size exclusion (SEC, and hydrophobic interaction (HIC) chromatography for the characterization and quantification of biopharmaceuticals. Several strategies which can be employed with these chromatographic techniques such as salt and pH gradients will be considered as well as the role of UHPLC.
View the entire tutorial »
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CHROMacademy Quick Guides
- Reversed Phase HPLC for the Analysis of Biomolecules
CHROMacademy eLearning Modules
- Introduction to Ion Chromatography
- Fundamentals of MS Proteomics Research
CHROMacademy Webcasts & Tutorials
- Techniques Employed in Biopharmaceutical Analysis - Part I Reversed Phase and HILIC
- Introduction to Ion Chromatography
- HPLC Techniques in Biopharmaceutical Analysis
- Characterization of Protein Biopharmaceuticals
- The Analysis of Post Translational Modifications Using LC-MS/MS
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