Solving retention time and selectivity changes in LCMS method transfer...
Column overload can lead to problems with chromatographic quantitation. Learn how to recognize symptoms of HPLC column overload and how to avoid it.
by Tony Taylor
These equations are really useful in the lab and I hope that you will be able to use them to improve the robustness of your separations...
The peaks in your chromatogram are split, broadened, and appearing at different retention times. Before righting off the column completely it may be possible to regenerate it...
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