EVENT OVERVIEW
Ion exchange chromatography (IEX) and size exclusion chromatography (SEC) are the main stay of the biopharmaceutical analyst's arsenal. These techniques provide much more information than merely charge variance and aggregation analysis. In this event we will discuss how, why, and where IEX and SEC are typically employed in biopharmaceutical characterization and quantification. Strategies for developing more robust IEX and SEC methods will be explored - this will include reducing secondary interactions in SEC, examining the effect of temperature and pressure on SEC separations, comparing the use of pH and salt gradients in IEX, and discussing the role of UHPLC. Finally, an introduction to the use of hydrophobic interaction chromatography (HIC) in biopharmaceutical analysis will be given.
