calendar24 February 2016
Volume 12 Issue 3
The Column

24 February 2016 Issue

Look inside for practical advice on separation science and thought–provoking commentary.

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The 2015 Instrumental Innovations

Characterizing biological processes in single cells with CE

The characterization of transcripts, proteins, peptides, and metabolites in cells is important to study disease mechanisms and develop novel therapeutics. Peter Nemes — from the George Washington University, in Washington, USA — spoke to The Column about the important role of CE–ESI and TOF-MS in this area of research.

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The benefits of TOF-HRMS in bioanalysis

Quantification of mycotoxins in peanut butter using SFE–SFC–MS

An on-line SFE–SFC–MS approach was successfully used to extract and analyze aflatoxin B1 and B2 from spiked peanut butter samples.

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Finding the right calibration standards in GPC/SEC

Detecting choline using IC

This article describes the performance of the AOAC Official First Action Method (AOAC Method 2012.20) chosen by SPIFAN, which uses microwave-assisted hydrolysis sample preparation followed by ion chromatography (IC) separation with suppressed conductivity detection for the determination of choline.

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The power of planar chromatography

Are you an experienced method developer?

Incognito offers some advice that is not to be taken lightly!

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Improving UHPLC method performance

Analysis of FAMEs using GC–VUV

The powerful characteristics of gas chromatography (GC) with VUV (vacuum ultraviolet) detection for the analysis of saturated and unsaturated fatty acids are described.

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Trends in bioanalysis using LC–MS–MS

News update: Using UHPLC–MS–MS to analyze drug use profiles

The analysis of honeybee venom, identifying drug use patterns between nightclubbers and criminals using UHPLC, and the latest news in brief are featured.

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