Troubleshooting and practical help for chromatographers
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The CHROMacademy biochromatography module will provide a thorough introduction to biopharmaceuticals. Written in conjunction with the Research Institute for Chromatography (RIC) and sponsored by Agilent Technologies.
A picture paints a thousand words. The art of GC troubleshooting often lies in being able to recognize a problem from the evidence presented in the chromatogram or baseline appearance...
After stationary phase choice, organic solvent content has the greatest impact on analyte retention and separation in HILIC...
I’ve often written about the ‘lazy’ chromatography which has swept through our industry, whereby 0.1% (w/w or w/v!) TFA or Formic acid is used to ‘buffer’ the eluent system well away from the pKa of analyte molecules...
Solving retention time and selectivity changes in LCMS method transfer...