High-Resolution
Separations for Protein LC/MS
Barry
E Boyes1,2, Benjamin Libert1, Stephanie Schuster1,
Brian Wagner1, William Miles1, Joseph Kirkland1
1Advanced
Materials Technology Inc., Wilmington, DE; 2Complex Carbohydrate
Research Center, University of Georgia, Athens, GA
A poster by Advanced Materials Technology, presented at 2016 ASMS meeting this June in San Antonio, TX, described studies in which various mobile phase additives were compared for gradient LC-UV and LC-MS analyses of proteins, intact monoclonal antibodies and their fragments. Superficially porous particle (SPP, Fused-CoreÒ 400 Ångstrom and prototype 1000 Ångstrom) columns compared favorably with 300 Ångstrom totally porous columns for the analyses of protein mixtures, intact mAbs and their fragments using a new additive, difluoroacetic acid (DFA). Click here to download poster
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