Watch
this video to see how the CHROMacademy HPLC troubleshooter can help to
quickly solve the problem of split peaks in an HPLC chromatogram … and
pick up a few extra troubleshooting tips too.
Tech Tip - Investigating (and solving) robustness issues in HPLC
Not
every method is as we would hope it would be. Some methods come to us
in imperfect form and we have to ‘live with them’, whilst others are
difficult separations and by necessity need to be developed using close
control of several variables...
The
required time and conditions actually depend upon the polarity of the
stationary phase, the column length, internal diameter and thickness of
the stationary phase film and the required upper operating
temperature...
I
guess we have all had the issue at some time or other. A blank
injection of eluent (or a zero volume dummy injection) gives rise to
discreet peaks on the chromatographic baseline which may interfere with
analyte peaks...