Analysis of Cephalosporins on a Polar Endcapped C18 3 µm Particle Column
Valeria Barattini, Thermo Fisher Scientific
The analysis of species containing polar groups using reversed phase HPLC can be challenging. The polar groups present on the analytes can result in secondary interactions with silanols on the silica surface. Furthermore, the overall polarity of the molecule sometimes calls for the use of mobile phases with very high aqueous content to ensure the analyte solubility, retention, and elution, at the expense of stationary phase stability. In this application note we demonstrate the analysis of a mix of four analytes from the cephalosporin antibiotics family on a Thermo Scientific™ Syncronis™ aQ 3 µm particle column. The analysis yields excellent peak shape and resolution in short run times, as well as outstanding reproducibility.
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An Improved HPLC Method for the Separation of Norethindrone and Mestranol with Progesterone as Internal Standard
Derek Hillbeck, Thermo Fisher Scientific
This work demonstrates the use of a Thermo Scientific™ Syncronis™ 3 µm C8 column to produce effective separation of norethindrone, progesterone, and mestranol giving equivalent results to those required from the USP method but in less time.
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