Please join us for a discussion about how to troubleshoot some of the more common and not so common problems that can arise when using analytical HPLC columns. Some of the topics covered will include how to mitigate issues related to chromatographic conditions, instrumentation, secondary interactions, and sample preparation. This presentation is intended to be used as a guide to resolving the complications that occur in the day-to-day use of HPLC and how to identify and avoid the most common sources of these problems.