Intact mass analysis of monoclonal antibodies (mAbs) via LC-MS methods can be accomplished in minutes. It is typically used to confirm the mass of the target mAb, and can also be used to profile product-related variants such as glycosylations.

LC-MS intact mass analysis of antibodies becomes a routine and high throughput analysis in light of the advancements made in LC-MS technologies and informatics. Using the provided LC-MS experimental conditions as well as data processing settings, high-quality, intact mAb data can be obtained routinely for mAb identification and relative glycoform quantitation.

This application note describes in detail the routine LC-MS analysis of intact antibodies using the Waters mAb Mass Check Standard.

Sincerely,
Waters Biopharmaceutical Team

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