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The Fundamentals of UV Spectroscopy

This course describes the basic theory of UV-visible spectroscopy, differences between instrument types, tips to help you make qualitative and quantitative measurements correctly, and example applications.

By taking this course, you will:

• Understand why molecules absorb UV-visible light and how the spectrum changes with molecular structure
• Know the advantages and disadvantages of different instrument types
• Learn how to select and use sample cells correctly
• Understand the possible causes of poor-quality measurements and deviations from Beer's Law
• Appreciate how to improve selectivity in quantitative applications
• Understand how spectroscopic instruments are calibrated and qualified

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