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Fundamentals of UV-visible Spectroscopy
Instructor: Mark Powell, PhD
This course describes the basic theory of UV-visible spectroscopy, differences between instrument types, tips to help you make qualitative and quantitative measurements correctly, and example applications.
By taking this course, you will:
- Understand why molecules absorb UV-visible light and how the spectrum changes with molecular structure
- Know the advantages and disadvantages of different instrument types
- Learn how to select and use sample cells correctly
- Understand the possible causes of poor-quality measurements and deviations from Beer's Law
- Appreciate how to improve selectivity in quantitative applications
- Understand how spectroscopic instruments are calibrated and qualified
Course structure:
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Session 1: Fundamentals
 The electromagnetic spectrum
Ultraviolet and visible radiation
Electronic transitions and the UV spectrum
Chromophores, conjugation, and auxochromes
Empirical rules for calculating absorption maxima
Shifts in absorption maxima (intensity and wavelength)
The Beer-Lambert Law (and deviations from it) |
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Session 2: Instrumentation
Components of a UV–visible spectrometer
Light sources and detectors
Single-beam and double-beam instruments
Resolution and sensitivity
Scanning and diode array instruments Sample cell (cuvette) materials
Care and use of sample cells
Sample cells for special applications |
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Session 3: Qualitative and Quantitative Methodology
Solution preparation and choice of solvent
Compound identification and purity evaluation
Choice of quantitation wavelength
Calibration
Complexation techniques
Derivative spectrometry
Quantitative measurements in multi-component systems
Photometric titrations |
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Session 4: Applications
Measurement of reaction rates and equilibria
Assay of metal ions in water
Enzymatic assay of sugars
Structural elucidation of a degradation product
Assay of diflunisal in Diflunisal Tablets USP
Measurement of dissolution rate
Quantification of pollutants in stack emissions
Analysis of polymer additives |
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| Contact Glen Murry at 732-346-3056 or Gmurry@Advanstar.com. |
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