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Strategies for Upstream Intensification: The Seed Train

Upstream processes can be intensified using a number of strategies which can deliver significant improvements in speed, flexibility and reliability. In this white paper, we explore two of these approaches: high cell density cryopreservation (HCDC), and the use of a perfused seed train including a specially designed expansion medium.

Specially designed expansion medium for your perfused seed train

Our new Cellvento^® 4CHO-X Expansion Medium supports cell expansion including N-1 perfusion for Chinese Hamster Ovary (CHO) cell lines. It is formulated with a high nutrient concentration to achieve low cell specific perfusion rates (CSPRs) at high cell densities. The medium is chemically defined, of non-animal origin, and contains no hydrolysates or components of unknown composition.

Best Regards,
Your Merck Team

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