Separation Science: The State Of The Art
October 14 • October 15 • October 18
Day 1
Day 2
Day 3
Emerging Trends in Liquid Chromatography
Emerging Trends in Hyphenated Techniques
Emerging Trends in Sample Preparation and Column Technology
Thursday, October 14, 2021 8am EDT | 1pm BST | 2pm CEST
Friday, October 15, 2021 8am EDT | 1pm BST | 2pm CEST
Monday, October 18, 2021 8am EDT | 1pm BST | 2pm CEST
Event Overview
A special three-day virtual symposium organized by LCGC in collaboration with The Chromatographic Society (ChromSoc) and the Royal Society of Chemistry’s Separation Science Group (RSC SSG). The symposium will be divided into sessions covering state-of-the-art advances in core areas, including liquid chromatography, hyphenated gas- and liquid chromatography, sample preparation, and column technology, and their role in key application areas. The symposium will commence each day with a plenary lecture from an established pioneering scientist and close with a session from a separation scientist who is regarded as an innovative emerging leader. A vital component of this “must attend” virtual event is the State-of-The-Art Technology Sessions where leading companies demonstrate how the latest “state-of-the-technologies” they have developed benefit the separation scientist in practice.
Learn about the latest advances in liquid chromatography, hyphenated techniques, column technology, sample preparation, and important application areas
Find out how the latest state-of-the-art technologies from leading separation science companies benefit the separation scientist in practice
Discover how the latest state-of-the-art technologies and novel advances can help separation scientists achieve the results they are looking for
Moderator
Alasdair Matheson Editor-in-Chief LCGC Europe
Thursday, October 14, 2021 at 8am EDT | 1pm BST | 2pm CEST
AGENDA
FIRST SESSION: LIQUID CHROMATOGRAPHY
8:00am – 8:45am EDT PLENARY LECTURE Current Speed and Efficiency Limits of Liquid Chromatography and Future Prospects
The plenary lecture to kick off this special virtual symposium will explore the current limits in speed and efficiency of liquid chromatography separations. This will be done using “so-called” kinetic plot representations. The application areas where ultrahigh pressures and small particles are most useful will be discussed in relation to the instrument quality (extracolumn volumes, injector and detector contributions). Emphasis will be put on the selection of the column length with which one can benefit most from the available ultrahigh pressures. It will be shown that the applications where the use of ultrahigh pressures is most advantageous are currently situated in the range of high efficiency separations, that is, the range where long columns rather than short columns are needed. Despite its high degree of maturity, the technique still does not deliver the required separation power needed to unravel the complex samples encountered in the state-of-the-art research in biology and drug development, for example, proteomics and metabolomics, or in contemporary food and environmental analysis. There is therefore a vivid search ongoing to find a solution to enable the current performance limits to be surpassed. These involve the use of even higher pressures, the use of perfectly ordered columns produced using silicon micromachining or 3D-printing, the use of particles with engineered mesopores, and innovative instrument designs. An overview of these state-of-the-art developments will be given as well.
Gert Desmet Full Professor Vrije Universiteit Brussel (VUB), Brussels, Belgium Read More
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Gert Desmet has a master’s degree and Ph.D. in chemical engineering from the Vrije Universiteit Brussel (VUB) in Brussels, Belgium, where he is currently a Full Professor in Chemical Engineering. His research is mainly focused on the miniaturization and automation of separation methods, as well as on the investigation and the modelling of flow effects in chromatographic systems. He is a past chair of the Chemistry Panel of the Flemish National Science Fund and is currently the Deputy-Director of the Solvay Institute for Chemistry. He is also an associate editor for the journal Analytical Chemistry and holder of an ERC Advanced Grant. In 2008, he received the Emerging Leader in Chromatography Award from LCGC. In 2009, he received the Silver Jubilee Medal of the Chromatographic Society of the UK, and in 2019 he received the American Chemical Society Award for Chromatography. He is currently a member of the permanent scientific committee of the international HPLC and ISC conference series.
8:45am –9:00am EDT STATE-OF-THE-ART TECHNOLOGY SESSION 1 Enhance Your Data and Go Beyond UV with Mass Detection
While ultraviolet detection is a well-established and reliable LC detection method, it requires a standard reference for reliable identification of compounds, and trace impurities may be too low in concentration to be detected. To improve upon this method, mass spectrometry can be utilized as a mass detector, when included after UV, to identify and quantify peaks separated from the LC. Furthermore, selected ion monitoring (SIM) can be used to separate compounds in co-eluting peaks. Agilent’s LC/MSD iQ is a small, robust, and powerful single quadrupole mass detector that can fit into any LC stack similar to any other detector. An analysis of Trazodone has shown detection limits at sub-ppb levels, well beyond the detection limits of the DAD UV detector. A reaction monitoring analysis was carried out, showing that the LC/MSD iQ detected many compounds that eluded the UV detector. Several side reaction products were also identified, and some insight was gained into the reaction mechanism from the mass information.
Kyle Covert, PhD LC/MS Applications Scientist Agilent Technologies Read More
Kyle Covert has an extensive background in mass spectrometry; from building TOF instruments at synchrotrons, to peptide synthesis and de novo sequencing using an old Finnegan TSQ. He has worked at Agilent for the past 3 years, developing workflows for LC/SQ and OpenLab CDS. He enjoys experimenting with new recipes and cooking with his 4-year-old daughter.
9:00am – 9:30am EDT Cracking the Code of Novel Drug Modalities by Using Innovative Analytical Approaches
The presentation will discuss the analytical challenges associated with the analysis of a broad range of new modalities with extended molecular space and physicochemical properties that require innovative analytical approaches, such as UHPLC–UV–MS and multidimensional LC–MS, such as HILIC and reversed-phase LC/IP-reversed-phase LC. This presentation will cover developments in both biopharmaceutical and pharmaceutical analysis.
Alex Goyon Senior Scientist Genentech Research and Early Development (gRED) Read More
Dr Alexandre Goyon is a Senior Scientist in the Genentech Research and Early Development (gRED) organization. He received a Ph.D. degree in pharmaceutical analytical chemistry in 2019 from the University of Geneva, in Switzerland. He has published 30 peer-reviewed papers and he is the first author of 17 of them. His team supports early- and late-stage research.
9:30am – 9:45am EDT
BREAKOUT SESSIONS/PRODUCT SHOWCASE/VIRTUAL BOOTH PRESENTATION
9:45am – 10:00am EDT
LIVE PANEL DISCUSSION Live QA discussion with speakers from session one answering questions from the audience
SECOND SESSION: LIQUID CHROMATOGRAPHY
10:30am – 11:00am EDT Novel Microchip Designs for Establishing the Next Generation of Multi-dimensional LC
The tangible benefits of multidimensional liquid chromatography technology with respect to realizing a high peak capacity per unit time have led to a significant momentum for multidimensional LC instrument and application development. Aiming at reducing the number of zero-dead-volume connections, to make LC×LC more user-friendly while minimizing extracolumn dispersion, a microfluidic modulator chip compatible with a commercially available switching valve was designed to enable sample transfer between columns in a 2D-LC setup. Chip prototyping and proof-of-concept of the applicability of the microfluidic modulator chip is demonstrated in a heart-cut multidimensional proteomics analysis. To advance the resolving power the development of spatial 3D-LC is pursued. The concept, design aspects, chip prototyping aspects, and separation potential will be discussed.
Sebastiaan Eeltink Full Professor Vrije Universiteit Brussel (VUB), Brussels, Belgium Read More
Sebastiaan Eeltink received his Ph.D. degree in chemistry in 2005 from the University of Amsterdam, The Netherlands, for his dissertation “Packed and monolithic capillary columns for LC”. From 2005–2007 he conducted postdoctoral research at the University of California, Berkeley, USA, and he was a guest scientist at The Molecular Foundry in the Lawrence Berkeley National Laboratory (Department of Energy-funded nanoscience research facility). Here, he developed novel column formats, including coated capillary columns and monolithic structures in microfluidic chips. In 2007, he joined a leading manufacturer of high-tech analytical instruments. In 2009, Sebastiaan received the Odysseus Award from the Research Foundation Flanders to establish his research group within the Department of Chemical Engineering at the Vrije Universiteit Brussel. The Eeltink lab is internationally recognized for pushing the boundaries of performance limits in separation science and for exploiting new possibilities in chip technology to address challenging analysis problems. Sebastiaan Eeltink is a Full Professor and author of more than 100 scientific publications, three book chapters, and three patent applications on spatial three-dimensional liquid chromatography.
11:00am – 11:15am EDT STATE-OF-THE-ART TECHNOLOGY SESSION 2 At-line monitoring of impurities and critical quality attributes in biopharmaceutical up- and downstream processes using dedicated HPLC
Adeno associated virus (AAV) is the leading vector in the field of gene therapy because of its low toxicity, good overall safety profile, and ability to maintain stable expression for long periods of time. It is therefore crucial to develop a robust and high efficiency platform for its manufacturing. One of the key challenges in manufacturing viral vectors is to increase the ratio between empty and full capsids. The most efficient way is to design the USP to result in less than 10% of empty capsids. This can be realised by process optimisation using at-line HPLC to allow for analysis of the full and empty capsids ratio directly in the harvest. Fast and reliable in HPLC methods to allow for process optimisation and assessing the purity of the final product using PATfix system will be presented.
Ales Strancar Managing Director BIA Separations d.o.o. Read More
Ales Strancar is executive managing director of BIA Separations since funded in 1998. Ales is one of the main inventors of the CIM Convective Interaction Media® monolithic columns technology and co-inventor of many analytical methods and purification processes including pDNA, mRNA, AAV, Adeno and other viruses. Ales is author or co-author of more than 90 scientific papers dealing with separation and purification technologies. He is a co-author of several granted USA patents and their foreign equivalents in the field of biomolecule separations and purification.
11:15am – 11:45am EDT Ultra-Flow-Flow Liquid Chromatography for Single-Cell Analysis
Human tissues contain a variety of cell types and subtypes with distinct functions, and understanding protein expression in tissue substructures and single cells is of great importance for biomedical research. Although LC–MS-based proteomic analyses are capable of quantifying thousands of proteins, the extension to single-cell studies has been largely ineffective. This is primarily due to protein and peptide losses during sample processing, as well as limitations in LC and MS instrumentation. This presentation describes improvements in each of these areas to make broad single-cell and nanoscale proteomic measurements a reality. This talk will focus on developing separations to maximize proteome coverage at and near the single-cell level. For example, nano-LC separations are performed using in-house packed narrow-bore columns with 20-µm inner diameters and operating at just 20 nL/min, which both increases ionization efficiency at the nanoelectrospray source as well as decreases chemical noise arising from trace contaminants from the LC mobile phase. Efforts to minimize dead volumes, employ autosamplers at this scale, and reduce sample loading times will be detailed. To further increase proteome coverage beyond the limitations of a 1D separation, we have developed nanowell-mediated 2D-LC, where high-pH reversed-phase nano-LC separations are fractionated into microfabricated nanowells for subsequent low-pH nano-LC. This method was used to identify an unprecedented ~6000 proteins from low-nanogram biological samples. Finally, 2D separations by coupling nano-LC with gas-phase separations (drift-tube ion mobility spectrometry [IMS] and field asymmetric IMS) to identify an unprecedented ~1000 proteins from single mammalian cells, potentially transforming biomedical research and the understanding of biological systems on a new level, are described.
Ryan Kelly Associate Professor Bingham Young University, USA Read More
Dr. Kelly is an Associate Professor in the Department of Chemistry and Biochemistry at Brigham Young University (BYU), USA. He received his Ph.D. in analytical chemistry in 2005 from BYU and spent the next 13 years at Pacific Northwest National Laboratory where he began as a postdoc and ultimately served as Chief Technologist for the EMSL, a national scientific user facility, before returning to academia in 2018. A central theme of Dr. Kelly’s research has been the development of new technological solutions for improved biochemical analyses, including mass spectrometry-based omics. Dr. Kelly has authored or coauthored more than 100 publications and is a named inventor on 14 issued and pending patents, several of which have been licensed and commercialized. His work has been recognized with several awards including two R&D 100 Awards, a Federal Laboratory Consortium Award for Excellence in Technology Transfer, and the Georges Guiochon HPLC Faculty Fellowship.
11:45am – 12:00pm EDT STATE-OF-THE-ART TECHNOLOGY SESSION 3 Micro Pillar Array Column (µPAC™) Technology – Pushing Peptide Mapping with Chromatography
nanoLC-MS has been at the forefront of peptide mapping applications in Life Science research and biotherapeutic drug development during the last 20+ years. During this time, technology improvements on mass spectrometry and nanoLC, both instruments and software, have allowed researchers to identify more proteins, from more complex samples with more confidence in their results. With the µPAC™ nanoLC and capLC columns, PharmaFluidics has build on the initial work from Prof. Gert Desmet and Prof. Wim Demalsche (Free University of Brussels, Belgium), and created an innovative backbone for low flow chromatography columns. Using the micro pillar array approach, the µPAC™ columns are designed for extra-high resolution separations, offering significantly improved column robustness and reproducibility. Particularly eye-catching is the much reduced back pressure under which the µPAC™ columns are operating. This allows for separation paths of 50 cm up to 200 cm, offering the extra resolution required to characterize, qualify and quantify the peptides and proteins in the typically highly complex samples that are analysed. In this presentation, we will address the capabilities of the µPAC™ columns, and show examples of how the micro Pillar Array Column technology can be used in a variety of peptide mapping applications.
Robert van Ling Global Customer Success Manager PharmaFluidics Read More
Robert van Ling started just over 25 years ago at LC Packings, one of the front runners in nano and capillary chromatography. During these years, he was part of the team that introduced the PepMap columns and the UltiMate nanoLC and 2D LC systems. After acquisitions by Dionex Corp. (2000) and later Thermo Fisher Scientific (2012), his role expanded into market development of chromatographic methods for small and large molecules, with a keen interest in the peptide mapping and RNA analysis for biotherapeutics. In 2018, Robert joined PharmaFluidics and is an avid advocate for the exciting µPAC™ technology for LC/MS.
12:00pm – 12:30pm EDT RISING STAR OF SEPARATION SCIENCE: Developments in Comprehensive 2D-LC with Machine Learning, Chemometrics, and Data Handling
Comprehensive two-dimensional liquid chromatography is an extremely powerful technique that is rapidly becoming indispensable for the analysis of complex samples, because it (i) profits from a potentially much higher peak capacity than 1D-LC, (ii) exposes two chemical properties of the sample if the two separation modes are sufficiently different (that is, “orthogonal”), (iii) allows highly complex samples to be separated, and (iv) hyphenates separation modes with normally incompatible detectors, potentially providing additional sensitivity and selectivity. Despite all of its favourable traits, scientists and manufacturers struggle to bring the technique into industrial analytical laboratories. While GC×GC is a successful, robust, and established technique for the analysis of complex volatile mixtures, the potential that LC×LC offers is often shrouded by a widespread perception that the technique is not sufficiently mature and that its application is impeded by a seemingly unsurmountable range of challenges. Two challenges are the complex method development associated with 2D-LC and the efficient analysis of the data obtained. To extract all valuable information from what has been referred to by some as “a tsunami of data” or, more generally, “Big Data”, efficient data analysis strategies are evidently needed. This presentation will explore the possibilities of automating method development and the potential role of machine learning.
Bob Pirok Associate Professor University Of Amsterdam Read More
Bob Pirok obtained his Ph.D. with the distinction cum laude from the University of Amsterdam in 2019, defending two books of research, and previously worked at Shell. He is currently tenure-track Assistant Professor at the University of Amsterdam and focuses on the application of chemometrics to analytical chemistry, with a special interest in method development and data analysis for multidimensional chromatography. Other areas of interest include retention modelling and (reaction) modulation techniques for LC×LC. Pirok is visiting research professor at Gustavus Adolphus College in the group of Professor Dwight R. Stoll. He is also visiting researcher in the group of Professor André de Villiers of the Stellenbosch University in South Africa. He has received a number of international recognitions, including a Shimadzu Young Scientist Award at HPLC2015 Beijing, the Young Scientist Award Lecture during the SCM-8 meeting in Amsterdam in 2017, the Csaba Horváth Young Scientist Award at HPLC2017 Prague, the Journal of Chromatography Award during the ISCC conference in Riva del Garda in 2018, and the SCM Award at the SCM-9 meeting in Amsterdam in 2019. Pirok is the writer of the recently granted NWO ENW-PPS TA PARADISE proposal coordinated by PI Professor Arian van Asten. He was selected as an early career board member for the ACS journal Analytical Chemistry, starting from 2021.
12:30pm – 12:45pm EDT STATE-OF-THE-ART TECHNOLOGY SESSION 4 Smart Software Solutions for Multiple Heart-Cutting 2D-LC with Agilent MassHunter 11
When highest separation power is needed, 2D-LC is the method of choice. 2D-LC has been used successfully by many industries and for many applications such as biop harma and pharma, chemicals, food analysis, environmental samples and academic research. For the highest level of information, 2D-LC is often combined with MS detection. Agilent has recently introduced an integrated solution for Q-TOF detection with MassHunter 11. In this talk, we will give you a practical overview on the new 2D-LC Software. Using (bio)pharmaceutical samples, we will demonstrate how to set up and develop methods quickly and easily for time-based and peak-based heart-cutting measurements. We will show, how to analyze 2D-LC/MS data including spectral information. New features for increased productivity are presented.
Dr. Ulrich Eberhardinger Product Manager Agilent Technologies Read More
Dr. Ulrich Eberhardinger studied chemistry and biochemistry at the University of Stuttgart, Germany and earned his PhD in physical chemistry. He works for Agilent since 2002 and has been responsible for customer collaborations and the development and market introduction of various high-end software, hardware and application products and solutions in liquid chromatography and microfluidics. As the product manager for Agilent InfinityLab 2D-LC Solutions, he shapes the future of multidimensional chromatography at Agilent.
Dr. Stephan Buckenmaier Principal Scientist Agilent Technologies Read More
Dr. Stephan Buckenmaier has received his PhD from the University of the West of England (Bristol, UK) in 2004. Following a Postdoc at the Proteome Center at the University of Tübingen, Germany, he joined Agilent in 2006. Currently, Stephan holds a position as Principal Scientist in Research and Development in the Liquid Phase Separation Division located in Waldbronn, Germany. His primary focus is on the development of 2D-LC workflow solutions and collaborations with external companies and academic institutions.
1:00pm – 1:15pm EDT
12:45pm – 1:00pm EDT
VENDOR BREAKOUT SESSIONS/PRODUCT SHOWCASE/VIRTUAL BOOTH PRESENTATION
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Adrian Clarke Analytical Network Leader Novartis Technical R&D (TRD) and Chemical and Analytical Development (CHAD)/ and Committee Member of The Chromatographic Society Adrian Clarke is the Global Analytical Network Leader in Novartis Technical R&D (TRD) and Chemical and Analytical Development (CHAD). His main responsibilities are identifying the strategic, operational, and technological needs of TRD analytics; defining, developing, and implementing innovative technologies and global business processes in TRD; and improving operational excellence and ensuring state-of-the-art analytics, as well as scientific and regulatory standards. His special interests are liquid-phase separations and hyphenated techniques (fast LC/UHPLC, 2D-LC, SFC, MS), column characterization, method development, and also analytical control and regulatory strategies, including mutagenic impurities. Adrian obtained his DPhil in analytical chemistry (separation sciences) at the University of York, UK. Following this he joined AstraZeneca (UK) in 1998 and worked in the analytical chemistry section of Process R&D. In 2010 he joined Novartis Pharma AG, Inhalation Development (Basel, Switzerland) as an analytical expert in drug substance analytics. In 2012 he moved into Chemical and Analytical Development as an analytical expert and Global Analytical Network Leader. Adrian has been a committee member of the Chromatographic Society, UK, since 2009, and is involved in international outreach activities with other societies and groups, organizing summer studentships; he has co-organized around 10 successful scientific meetings (including the state-of-the-art/emerging technologies series). He has a strong publication record and is an invited guest editor for several scientific publications, including LCGC Europe.
MODERATOR
Friday, October 15, 2021 at 8am EDT | 1pm BST | 2pm CEST
FIRST SESSION: EMERGING TRENDS IN HYPHENATED LC TECHNIQUES
8:00am – 8:45am EDT PLENARY LECTURE Advances in Liquid Chromatography with Ion Mobility-Mass Spectrometry
In the past decade, ion mobility and especially ion mobility hyphenated with (high-resolution) mass spectrometry (IM-MS) has become of increasing interest to separation scientists for addressing a diverse range of analytical challenges. Of particular importance to various ‘omics fields and related small-molecule applications has been the introduction of IM-MS instrumentation that allows a generic separation of all ions entering the mass spectrometer. This presentation will highlight the complementary role that ion mobility technology plays with respect to both chromatography and mass spectrometry within hyphenated workflows. Of key importance is that IM separation can provide (i) an additional and complementary signal for the alignment of chemically related signals, and (ii) the option to convert the measured arrival time into a new molecular descriptor (collision cross section, [CCS]) for confirming the identity of unknown compounds. Recent application examples of IM-MS demonstrating the realization of both of these aspects will be highlighted with a focus on the ruggedness of IM separation against matrix effects (for example, food analysis), the potential of advanced IM-enabled data independent analysis (DIA), and the prospects of using CCS as a complementary identification point for routine analysis.
Tim Causon Assistant Professor University of Natural Resources and Life Sciences, Vienna (BOKU), Austria Read More
Tim Causon is Assistant Professor and Deputy Head of the Institute of Analytical Chemistry at the University of Natural Resources and Life Sciences (BOKU), Vienna, in Austria. His main areas of research are separation sciences, ion mobility-mass spectrometry, quantitative metabolomics, and analytical method development for diverse small-molecule applications including studies of ion formation processes, metabolomics for biotechnology (microbial cell factories), and phenolic profiling for studying traditional winemaking practices. He is currently a mentor of a nationally funded research project (FWF Lise Meitner Program) and is a member of the editorial advisory board of the international journal Analytica Chimica Acta and of the training committee of the Metabolomics Society.
8:45am –9:00am EDT STATE-OF-THE-ART TECHNOLOGY SESSION 1 Research projects and collaborations at PSS for the advancement of GPC/SEC
GPC/SEC is the key technology to investigate structure-property-relationships of synthetic and natural macromolecules. Although the concept of GPC/SEC was developed in the late 50s, it is currently a hot topic. Advancements for the analysis of biotherapeutics have led to an increasing interest in this separation technique. Demands for new routes to recycle synthetic polymers and for options of greener solutions have stimulated the development of modern solutions. PSS, as the specialist for liquid chromatography of macromolecules, is fully dedicated to the advancement of GPC/SEC within its own R&D department and with partners from universities and research institutes. This presentation will give a brief overview of the status of PSS research activities highlighting some projects from hyphenation with spectrometric and spectroscopic detection options to new stationary and mobile phases.
Daniela Held Director Marketing & Sales PSS GmbH Read More
Daniela Held studied polymer chemistry (PhD) in Mainz (Germany) and obtained her PhD in physical chemistry in 2000. In the same year she started working in the PSS software and instrument department, where she was responsible for software design and the development of turn-key systems. In 2007 she progressed to the PSS Global Marketing department. Additional responsibilities include education and customer training. In 2020 she was promoted to PSS General Manager and a member of the PSS Executive Committee.
9:00am – 9:30am EDT Recent Developments in Two-Dimensional Liquid Chromatography
The ever-present thirst for improved performance in liquid chromatography continues to fuel growth in the development and application of two-dimensional liquid chromatography (2D-LC). In some application areas, such as the ‘omics’ fields, the performance metric of interest is peak capacity, or the ability to separate many compounds. In this exciting area of research, 2D-LC has clear potential advantages over conventional one-dimensional liquid chromatography (1D-LC) because 2D-LC separations can yield a target peak capacity in shorter analysis times compared to 1D-LC separations. In other areas, separation speed and simplicity are most important. For example, analysis of a small number of target compounds in a complex mixture can be made much easier using 2D-LC than is possible by 1D-LC. In this presentation a leading pioneer in this field will briefly review these foundational concepts and illustrate their importance using contemporary applications from multiple fields. Dwight will then discuss advances in 2D-LC technologies in recent years, using application examples to illustrate their impact. The field is very much alive at the moment with important developments of both instrument technologies and workflows to simplify method development and data analysis. Finally, some areas of 2D-LC where further research is most acutely needed to move the field forward will be suggested.
Dwight Stoll Professor Gustavus Adolphus College in St. Peter, MN Read More
Dwight Stoll is a Professor of Chemistry at Gustavus Adolphus College in St. Peter, Minnesota, USA. He has authored or coauthored many peer-reviewed publications and five book chapters in separation science and speaks internationally on the topic. His primary research focus is on the development of two-dimensional liquid chromatography (2D-LC) for both targeted and untargeted analyses. Within this area he has made contributions on many aspects of the technique, including stationary phase characterization, biopharmaceutical analysis, new 2D-LC methods and instrumentation, and fundamental aspects, including re-equilibration in gradient elution liquid chromatography and analyte focusing. He has taught several short courses on 2D-LC in recent years at venues including Pittcon and the international HPLC20XX series. He is the 2011 recipient of LCGC’s Emerging Leader in Chromatography Award, the 2015 recipient of the American Chemical Society Division of Analytical Chemistry Award for Young Investigators in Separation Science, and in 2017 he received the Georges Guiochon Faculty Fellowship.
9:30am – 09:45am EDT
SECOND SESSION: EMERGING TRENDS IN HYPHENATED GC TECHNIQUES
10:15am – 10:45am EDT Comprehensive 2D GC Instrumentation: The State of The Art and Future Prospects
Comprehensive two-dimensional gas chromatography (GC×GC) was introduced 30 years ago and can now be considered as a rather well-established technique. Across three decades of history, there has been considerable instrumental evolution, in particular (though not only) involving forms of modulation and use of mass spectrometry (MS) devices. The present lecture intends to provide an updated view on the most common, as well as the most innovative/interesting modulation approaches, and on the three-dimensional hyphenated technique, GC×GC–MS. A view on possible future instrumental scenarios is also given.
Peter Tranchida Associate Professor University of Messina, Messina, Italy Read More
Peter Q. Tranchida currently occupies a position as Associate Professor in Food Chemistry in the Department of Chemistry, Biological, Pharmaceutical, and Environmental Sciences at the University of Messina, Italy. His research activities are focused mainly on the study of complex food (and non-food) samples by using advanced chromatography processes, in particular classical multidimensional and comprehensive two-dimensional chromatography systems. Specifically, he has performed a great deal of research work in the field of heart-cutting multidimensional gas chromatography and liquid-gas chromatography, as well as comprehensive 2D gas chromatography (GC×GC). In terms of mass spectrometry systems, he currently uses rapid-scanning single quadrupole, triple quadrupole, and low- and high-resolution time-of-flight devices. In the field of GC×GC, he has performed applications, method optimization studies, and introduced novel approaches within the context of flow modulation. In 2012, at the 9th GC×GC symposium, held in Riva del Garda, Italy, he was awarded the John Phillips Award for his achievements in the field of GC×GC. In 2019, at the 16th GC×GC symposium held in Fort Worth, Texas, USA, he was awarded the GC×GC Lifetime Achievement Award.
11:00am – 11:30am EDT Exploiting Headspace High-Capacity Solid-Phase Microextraction and Gas Chromatography Dual Mass Spectrometric and Broadband Vacuum Ultraviolet Adsorption Detection to Identify Bacterial Species Under Altered Growth Conditions
Identification of pathogenic microorganisms in vivo (breath, tissue, biopsies) is critical to help fight infections. Several metabolites have been established as biomarkers for different species of bacteria. However, bacteria have developed stress responses to cope with variable conditions encountered in vivo. Since the microorganisms responsible for infectious diseases are likely under stressed conditions in vivo, identification could be hampered because stress responses are directly related to changes in several metabolomic pathways. Therefore, tracking additional biomarkers is essential to unequivocal identification. Three different bacteria, Pseudomonas aeruginosa, Bacillus cereus, and Staphylococcus aureus, were grown in vitro in non-stressed and stressed conditions (acidic, basic, oxidative, ethanolic, and saline conditions). In order to determine the ability to identify and differentiate between the microorganisms grown in normal and stressed conditions, samples were analyzed using headspace solid-phase microextraction prior to untargeted analysis with gas chromatography coupled with parallel detectors, mass spectroscopy, and vacuum ultraviolet spectroscopy. Results were then evaluated using multivariate statistical analysis (principal component analysis, hierarchical cluster analysis, partial least square discriminant analysis, and random forest). Based on reported volatile metabolites, bacteria grown in normal in vitro conditions were able to be differentiated using PCA. However, for altered stressed conditions, unequivocal identification of bacterial species required a combination of 96 compounds to differentiate between the bacteria with statistical tools.
Tiffany Liden Team Leader Researcher at the University of Texas at Arlington (UTA), USA Read More
Tiffany studied biochemistry at Texas Woman’s University and then completed her master’s degree in chemistry education at the University of North Texas. After graduation, she spent five years working as an adjunct professor at the Tarrant County College. She returned to school in 2015 and obtained a Ph.D. in chemistry from the University of Texas at Arlington (UTA). When she is not facilitating the success of students in the laboratory, she spends her time using separation science and mass spectrometry to solve a variety of analytical problems. Her research has centred around the evaluation of the efficacy of wastewater treatment technologies with Collaborative Laboratories for Environmental Analysis and Remediation (CLEAR), in addition to untargeted metabolomics using GC–MS and LC–MS/MS. She has 12 peer-reviewed publications and 14 presentations, posters, and invited talks.
11:30am – 12:00pm EDT RISING STAR OF SEPARATION SCIENCE: Untargeted Profiling and Differentiation of Geographical Variants of Wine Samples Using HS-SPME FM X GC–TOF-MS with the Support of Tile-Based Fisher Ratio Analysis
The volatile fraction of food, also called the food volatilome, is increasingly used to develop new fingerprinting approaches. The characterization of the food volatilome is important to achieve desired flavour profiles in food production processes or to differentiate different products, with winemaking being one popular area of interest. In the present research, HS-SPME coupled to FM GC×GC–TOF-MS was used to characterize geographical-based differences in the volatilome of five white wines of Sicilian origin. All wines were produced with the same vinification method in 2019. To minimize the influence of minor bottle-to-bottle differences, three bottles of the same wine were randomly selected and three samples were collected per bottle, resulting in nine sample replicates per wine. Particular emphasis was devoted to the operational conditions of a novel low-duty cycle flow modulator. A fast FM GC×GC–TOF-MS method with a modulation time of 700 ms and a re-injection period of 80 ms was developed. Following GC×GC–TOF-MS analysis, the instrumental software was exploited to identify class-distinguishing analytes in the dataset via a Fisher ratio calculation. A tile size of 10 modulations on the first dimension and 45 spectra on the second dimension was used to encompass average peak widths and to account for minor retention time shifting. An offline software was used to apply an ANOVA test. A p-value of 0.01 was applied to select the most important class-distinguishing analytes, followed by principal component analysis.
Mariosimone Zoccali Assistant Professor University of Messina, Messina, Italy Read More
Mariosimone Zoccali is an Assistant Professor of Analytical Chemistry in the Department of Mathematical and Computer Science, Physical Sciences, and Earth Sciences at the University of Messina in Italy. His research is focused on the development of multidimensional chromatographic instrumentation and software (GC×GC, LC–GC, LC–GC×GC, SFE–SFC), coupled to state-of-the-art MS for the study of complex matrices constituents and contaminants. Mariosimone has authored or coauthored more than 50 articles and book chapters. He has given award-winning presentations at multiple international meetings. He has been directly involved with the development of award-winning instrumentation. In particular, he assembled a five‐dimensional instrument consisting of a liquid chromatography system combined with a comprehensive two‐dimensional gas chromatography‐triple quadrupole mass spectrometry instrument. The results of that work were published in Analytical Chemistry. Dr. Zoccali received further recognition for his achievements in 2018 with the Young Researcher Award, on behalf of the Interdivisional Separation Science Group (Italian Society of Chemistry). He has pursued and won multiple travel awards to better disseminate his research to the broader scientific community. He is also leader of the working group 3 of the EuChemS-DAC Sample Preparation Study Group.
12:00pm – 12:15pm EDT
Sam Whitmarsh Head of Process Research and Development and Committee Member of the Royal Society of Chemistry’s Separation Science Group (RSC SSG) CatSci Sam was awarded his Ph.D. from the University of Bristol following work on the synthesis of novel steroid macrocycles and measurement of their complexes by high-resolution mass spectrometry with Professor Anthony Davis. In 2007, Sam joined Process R&D in AstraZeneca as an analytical scientist, leading analytical components of drug development across pre-clinical to Phase III drug candidate projects, working on analytical method QBD, trace genotoxic impurities, and novel approaches to high-throughput chromatographic analysis. In 2010, Sam moved to BP and carried out a range of technical and people leadership roles over the next 10 years. He led the formation of the high-resolution mass spectrometry petroleomics facility in the UK, managed a team of 35 staff, and left BP as global analytical expert, leading the BP Analytical Science Network. In 2020, Sam joined CatSci Ltd as the Head of Process Research and Development. He is passionate about enabling great scientists to do great science that exceeds the expectations of their customers and contributes to better outcomes for patients.
10:45am – 11:00am EDT STATE-OF-THE-ART TECHNOLOGY SESSION 3 From research to routine: Major advances in multi-dimensional GC
GC×GC first attracted attention in the late 1970s, but for many years its superior separation capacity was not seen as being worth the additional technical complexity. Thanks to greatly improved hardware and software, this has changed, and as a result GC×GC is now being used routinely. Some major improvements in GC×GC are highlighted in this presentation – specifically when coupled to time-of-flight mass spectrometry (TOF MS) – for greater insight in to sample composition, while automating workflows to ensure complex chromatograms can be compared in minutes instead of days, or even weeks. A case study on perfume authenticity is used to demonstrate how comprehensive data acquired using GC×GC–TOF MS with Tandem Ionisation can be processed in a single, automated workflow in ChromCompare+ software, highlighting subtle differences between samples to be uncovered quickly and easily.
Dr. Laura McGregor Product Marketing Manager, SepSolve Analytical Read More
Laura’s background is in analytical forensic science, and her Ph.D. (at the University of Strathclyde, UK) focused on the chemical fingerprinting of environmental contamination using GC×GC−TOF MS. Following roles in application support and product marketing for Markes International’s TOF MS and software portfolios, she joined SepSolve in October 2017, where she oversees marketing activities across the full product range.
Monday, October 18, 2021 at 8am EDT | 1pm BST | 2pm CEST
FIRST SESSION: EMERGING TRENDS IN SAMPLE PREPARATION
8:00am – 8:45am EDT PLENARY LECTURE Green Sample Preparation: Restoring The Truth
The concept of green analytical chemistry (GAC) was established soon after the introduction of green chemistry. It was subsequently formulated in the form of 12 main principles that expressed the willingness to care for the environment and human safety as part of the development and application of analytical procedures. Sample preparation is considered critical in achieving “green analysis”. This is because early sample preparation methods consisted of tedious and, more importantly, solvent-consuming procedures. Over the past two decades, research in the area of green and sustainable sample preparation has been outstandingly successful, but the discipline has not been effective enough at projecting its advances in external platforms. Private and industrial laboratories see sustainable or more eco-friendly analytical chemistry as an extra burden rather than a new opportunity for efficient, cost-effective, and socially responsible practice. A key opinion leader and innovator in the field will pinpoint missed opportunities and mistakes, discuss their impacts, and suggest ways to address them. Advances in sample preparation technologies will be juxtaposed with prevailing practices to allow a clear view of the solutions already available. Sample preparation is an important tier of environmental protection and a powerful tool to unfold major and complex ongoing issues, with implications for the sustainable development goals (SDGs). Restoring the truth about the greenness of the sample preparation step is critical in separation science, and scientists within and outside the research community need to acknowledge the great social responsibility and align with pollution abatement and sustainable development.
Elia Psillakis Full Professor Technical University of Crete, GR-73100, Chania, Crete, Greece Read More
Elia Psillakis is Full Professor in Water Chemistry at the School of Chemical and Environmental Engineering, Technical University of Crete, Greece, and the Head of the EuChemS-DAC Sample Preparation Study Group and Network. She is editor-in-chief of Advances in Sample Preparation by Elsevier, specialty chief editor of the Environmental Analysis specialty section in Frontiers in Analytical Science by Frontiers, and an associate editor of Journal of Separation Science by Wiley. Her research activities focus on (i) understanding the fundamentals and developing new sample preparation methods and (ii) monitoring the environmental fate of organic contaminants in natural and engineered systems. Her investigations have resulted in three patents, six book chapters, and close to 100 publications in ISI journals that have received more than 7400 citations (h-index = 44) and three “Top cited article” awards.
8:45am –9:00am EDT STATE-OF-THE-ART TECHNOLOGY SESSION 1 HiSorb sorptive extraction: Addressing analytical challenges and emerging trends
Modern laboratories find themselves under increasing pressure to balance new analytical challenges with increasing workloads, whilst accommodating demands to reduce the cost and improve the quality and quantity of information from each sample. The sample preparation process has been acknowledged as a major bottleneck, and with new emerging trends, such as sustainable extraction and automation, there is increased interest and scrutiny in this area of analytical chemistry. Here we show HiSorb, a high-capacity sorptive extraction technique for automated preconcentration of volatile and semi-volatile organic compounds, with analysis by thermal desorption–gas chromatography–mass spectrometry (TD–GC–MS). An alternative to liquid–liquid extraction (LLE) or solid-phase extraction (SPE), high-capacity sorptive extraction is a convenient approach for the analysis of complex samples. Applications include monitoring pollutants in environmental waters to ensure public safety, or profiling aromas and taints in foods for quality and authenticity.
Rachael Szafnauer Read More
Rachael Szafnauer received an M.Sci in forensic science from the University of South Wales, UK, where her final-year project focused on fingerprinting emerging psychoactive substances using advanced techniques such as GCxGC–TOF MS, in collaboration with Markes International. She later took up the role of thermal desorption product specialist at Markes, providing technical and application support to the commercial team, before taking on her current role as Product Marketing Manager for the Centri product line and specialising in the development of applications using extraction & enrichment techniques for GC-MS.
9:00am – 9:30am EDT Critical Considerations On Automated Sample Preparation
In this presentation current trends in sample preparation will be discussed. Attention will be paid to important consequences of automation, miniaturization and on-line hyphenation of sample preparation prior to analysis by gas chromatography, liquid chromatography and/or mass spectrometry. Practical examples will be shown that demonstrate how methods can be automated, while maintaining the performance criteria of classical methods used in environmental, food, fragrance and pharmaceutical analysis.
Frank David Principal Scientist Research Institute for Chromatography (RIC) Read More
Dr. Frank David received his Ph.D degree in 1986 at the Laboratory of Organic Chemistry of the Universiteit of Ghent under the direction of Prof. Dr. M. Verzele. Since October 1986, Frank David has been Principal Scientist at the Research Institute for Chromatography (RIC) in Kortrijk, Belgium. RIC is a private research company founded by Prof. Dr. Pat Sandra and offering analytical services and turn-key solutions based on chromatography and mass spectrometry to the industry and to private and governmental laboratories. Frank David is author of more than 100 scientific papers in different areas of separation science. His expertise includes capillary gas chromatography, supercritical fluid chromatography (SFC), GC–mass spectroscopy (GC–MS), multidimensional chromatography (GC–GC, GCxGC, LC–GC), and miniaturization and automation of sample preparation. Frank David is also consultant for instrument manufacturers and for industrial laboratories in the environmental, petrochemical, food, pharmaceutical, and chemical industries. In this function, he has a long experience in theoretical and practical training courses.
SECOND SESSION: EMERGING TRENDS IN COLUMN TECHNOLOGY
10:15am – 10:45am EDT Recent Technical Advances and Market-Drivers Shaping the Liquid Chromatography Landscape
There have been many advances in liquid chromatography (LC) columns over the past decade. Manufacturers continue to develop new and useful stationary phases of differing chemistry and bonding design that expand the utility of LC. Particle technology advances including architecture, alternative materials, as well as particle and pore size distribution control have continued to develop. Alternate column designs, such as micro-separation devices, have been commercialized and advances in hardware materials, particularly with the intent to increase sample pathway inertness, have surfaced. Additional market drivers have also played a major role in shaping the LC separations landscape. The focus on biopharmaceuticals, for example, has reignited interest in size-exclusion, ion-exchange, and hydrophobic interaction chromatography, among many others. This presentation will review and discuss some of the major technical advances and market drivers that have shaped developments in liquid chromatography products over the past several years. Perspectives on future trends will also be proposed.
Dave Bell LCGC Editor of “Column Watch” Restek Corporation, USA Read More
Dave Bell is a Director of Research and Development at Restek. He also serves on the editorial advisory board for LCGC magazine and is the Editor for the “Column Watch” series of articles. Over the past 20 years, Dave has worked directly in the chromatography industry, focusing his efforts on the design, development, and application of chromatographic stationary phases to advance gas chromatography, liquid chromatography, and related hyphenated techniques. His undergraduate studies in chemistry were completed at the State University of New York at Plattsburgh (SUNY Plattsburgh, USA). He received his Ph.D. in analytical chemistry from The Pennsylvania State University (State College, Pennsylvania, USA), and spent the first decade of his career in the pharmaceutical industry performing analytical method development and validation, employing various forms of chromatography and electrophoresis. Dave’s main objectives have been to create and promote novel chromatography (LC) technologies and to conduct research on molecular interactions that contribute to retention and selectivity in an array of chromatographic processes. His research results have been presented in symposia worldwide and have resulted in numerous peer-reviewed journal and trade magazine articles.
10:45am – 11:00am EDT STATE-OF-THE-ART TECHNOLOGY SESSION 2 Pore Controlled Technology to help you improve SEC method robustness and transferability for routine aggregate analysis of mAbs.
Size Exclusion Chromatography (SEC) is a well-established analytical method for quantitative and qualitative aggregate analysis of monoclonal antibodies (mAbs) and other related biotherapeutics. However, developing a robust, transferable SEC method for routine testing can be challenging for any analytical development laboratory. The root cause of the systemic failures and variability are less than ideal pore volumes. In this brief presentation, we discuss Pore Controlled Technology (PCT) and how that can improve method robustness and transferability for routine aggregate analysis of mAbs and other mAb formats.
Dr. Helen Whitby Bioseparations Specialist Phenomenex Read More
11:00am – 11:30am EDT Optimizing Efficiency in Temperature-Responsive Liquid Chromatography
An update on the latest findings on temperature effects in liquid chromatography.
Frederic Lynen Professor Ghent University Read More
Frederic Lynen professor and head of the Separation Science Group in the Department of Organic and Macromolecular Chemistry at Ghent University. His research comprises column technology, temperature responsive liquid chromatography, stationary phase synthesis, multidimensional separations, chromatographic predictive modelling and the development of LC-MS and GC-MS based non-targeted approaches allowing e.g. biomarker discovery. He authored over 140 journal articles in the field of separation sciences and was awarded the Jubilee Medal by the Chromatographic Society in 2019.
1:00pm EDT – 1:30pm EDT
Daniel Meston Research Scientist/PhD and Committee Member of The Chromatographic Society Vrije Universiteit Brussel, Belgium Dr Daniel Meston received his Ph.D. in 2020 from the Helmholtz Center for Infection Research in Germany, where his research focused primarily on the analysis of oxidative signalling in immune cells by LC–MS-based proteomics. Dr Meston currently works in the laboratory of Sebastiaan Eeltink and focuses on the development of novel technologies for LC–MS-based proteomics applications.
Dr Helen Whitby has been at Phenomenex since 2007. She is a senior technical specialist working in thetechnical department and specialize in Bioseparations and bulk and preparative chromatography. Before joining the technical department in 2017 she worked for 10 years in the UK sales team as the bulk and prep specialist and HPLC product manager for the UK sales team. Helen has a PhD from the University of Manchester working on analogues of Shikimic acid to treat malaria and prior to joining Phenomenex worked for 12 months in Chicago as a Post-doctoral researcher at the University of Illinois in Chicago on electron-deficient divalent reactive intermediates specifically nitrenium ions for the development of antiviral agents to treat Ebola.
11:30am – 11:45am EDT STATE-OF-THE-ART TECHNOLOGY SESSION 3 Research projects and collaborations at PSS for the advancement of GPC/SEC
11:45am – 12:15pm EDT RISING STAR OF SEPARATION SCIENCE: Latest Advances in 3D-Printing for Column Technology
In recent years additive manufacturing, or 3D-printing, has gained popularity as a fabrication tool in many fields. Over time, 3D-printing technology has advanced and it has been adapted to a broad range of techniques and materials. This could not stay unnoticed by the field of analytical chemistry. The availability and accessibility of 3D-printers make them an attractive alternative for producing column components, such as column cartridges, or customizing microfluidic housings with alternative formats to accommodate chromatographic beds. For analytical chemistry applications, the technologies used most commonly include the extrusion of melting thermoplastics (fused deposition modelling [FDM]), the fusion of polymer/metal powders (selective laser sintering [SLS]); selective laser melting (SLM), the polymerization of photocurable liquid resins (laser-assisted stereolithography [SLA]); digital light processing (DLP) and hybrid stereolithography. There are some uncommon approaches, such as two-photon polymerization. For example, reversed-phase LC separations were achieved by in-situ fabrication of porous polymer monoliths, directly within channels 3D-printed using FDM. Methacrylate monolithic columns were created in various polypropylene columns and inserted in 3D-printed column housings. SLM provides the opportunity to create new column housing, with designs of varying degrees of complexity and stronger materials. The use of titanium as a material with high thermal conductivity and suitable surface chemistry to attach monolith material within the housing was explored in the printed devices described. It can be used to thermally initiate polymerization for the fabrication of monolithic stationary phases within discrete regions of 3D-printed titanium devices. In this lecture, an overview will be presented of different 3D-printing techniques and materials that have been adapted to a range of applications for column technology.
Noor Abdulhussain Research Scientist/PhD Student University of Amsterdam, The Netherlands Read More
Noor Abdulhussain obtained her M.Sc. degree in 2017 from the University of Amsterdam. She is now a PhD student in the ERC STAMP (Separation Technology for A Million Peaks) project at the University of Amsterdam. In 2019, she was decorated with the best poster award at HPLC2019 (Milan, Italy), and was invited to give a lecture at the Emerging Separations Technologies Symposium (Chromatographic Society, London, UK). That same year, LCGC identified her as one of the rising stars in chromatography. While her current work mainly focuses on functionality assessments of 3D-printed devices for application to multidimensional separations, Abdulhussain remains involved in studying fundamental separation mechanisms, including hydrodynamic chromatography and size-exclusion chromatography. In January 2021 she founded the SistersinScience_NL Instagram platform together with two of her colleagues to show a glimpse of her work on social media and debunk stereotypes of chemists and other scientists. The platform resulted in much publicity and collaborations with groups, such as the Royal Dutch Chemical Society (KNCV), the Centre of Youth Communication Chemistry (C3), and the Royal Association of the Dutch Chemical Industry (VNCI).
12:15pm – 12:30pm EDT STATE-OF-THE-ART TECHNOLOGY SESSION 4 Micro Pillar Array Column (µPAC™) Technology – Pushing Peptide Mapping with Chromatography
12:30pm – 1:00pm EDT
