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Antibody Affinity Extraction for HCP Analysis

Ken Hoffman and Eric Bishop, Cygnus Technologies, LLC

Antibody Affinity Extraction (AAE) is a superior alternative to 2D Western blot for demonstrating host cell protein (HCP) antibody coverage and reactivity to those HCPs that persist through the downstream purification process.

Analysis of Cysteine-Linked ADC using HIC

Sonja Schneider, Agilent Technologies

Hydrophobic interaction chromatography (HIC) is used for the determination of drug-to-antibody ratio (DAR) of a cysteine-linked antibody drug conjugate (ADC).

TOYOPEARL GigaCap^® Cation Exchange Resins for Aggregate Removal from Monoclonal Antibodies

Tosoh Bioscience LLC

The data presented here demonstrate the effectiveness of TOYOPEARL GigaCap S-650M, TOYOPEARL GigaCap S-650S and TOYOPEARL GigaCap CM-650M resins in the elimination of mAb aggregates from protein A purified material.