Antibody Affinity Extraction for HCP Analysis

Ken Hoffman and Eric Bishop, Cygnus Technologies, LLC

Antibody Affinity Extraction (AAE) is a superior alternative to 2D Western blot for demonstrating host cell protein (HCP) antibody coverage and reactivity to those HCPs that persist through the downstream purification process.

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Effective Removal of mAb Aggregate Using Ca⁺⁺Pure-HA® (Hydroxyapetite) Media with Potassium Salts

Tosoh Bioscience LLC

A purified mAb sample, post-protein A purification step was loaded onto a Ca⁺⁺Pure-HA (Hydroxyapetite) column. Using potassium phosphate buffer as a loading buffer and potassium chloride as an elution buffer, the mAb aggregate content was reduced from 6.6% to as low as 1.3%.

Analysis of Cysteine-Linked ADC using HIC

Sonja Schneider, Agilent Technologies

Hydrophobic interaction chromatography (HIC) is used for the determination of drug-to-antibody ratio (DAR) of a cysteine-linked antibody drug conjugate (ADC).