Build your biopharma development and manufacturing knowledge base with the latest whitepapers, application notes, posters, and other educational resources from biopharma industry suppliers.

Host Cell Protein Analysis in Biologic Drug Development

Jennifer S. Chadwick, Robert Garnick, Chen Li, Shiaw-Lin Wu, BioAnalytix

This whitepaper reviews fundamental aspects of host cell proteins (HCPs) that pertain to biologic drug development and presents current and emerging approaches to HCP analysis.

Fast Track Formulation pH Screening with Automated Buffer Exchange on Big Tuna

Unchained Labs

The critical process of screening formulation buffers to optimize stability is labor intensive and time consuming, which are often limiting factors in biologics development. The conformational, chemical, and colloidal stability of a protein are strongly influenced by the buffer solution.

Fully Automated Digestion, Separation, and Analysis of the Human Prolactin Receptor Transmembrane Protein by LC-MS/MS

Shimadzu

Detection and quantification of peptides from transmembrane proteins is important for detection of biomarkers and new protein-based drugs. This paper presents a fully automated online digestion and liquid chromatography-mass spectrometry-multiple reaction monitoring (LC-MS/MRM) analysis of two model transmembrane proteins, a method that improves reproducibility and decreases chances of contamination.

Antibody Drug Conjugate Purification Using TOYOPEARL^® PPG-600M Resin

Tosoh Bioscience LLC

In this application note, antibody drug conjugates (ADCs) were separated into groups with low, medium, and high drug-to-antibody ratios (DARs) using TOYOPEARL PPG-600M resin with a three-step gradient.

Purifying Humira Biosimilar with Mixed-Mode Resins

Randy Drevland, Chelsea Pratt, Payal Khandelwal, Laura Moriarty, Elizabeth Jordan Dreskin, Bio-Rad

Remove the bottleneck of purifying therapeutic monoclonal antibodies (mAbs) for screening. Save time and increase productivity by automating multicolumn purification during drug development on the NGC Chromatography System.

Antibody Affinity Extraction for HCP Analysis

Ken Hoffman, Eric Bishop, Cygnus Technologies, LLC

Antibody affinity extraction (AAE) is a superior alternative to 2D Western blot for demonstrating host cell protein (HCP) antibody coverage and reactivity to those HCPs that persist through the downstream purification process.

Purification of Large Biomolecules with New Anion Exchange (AEX) Resin

Bio-Rad

IgM antibodies are one of the fastest growing groups of diagnostic and therapeutic candidates. Unlike IgGs, IgMs cannot be purified by affinity chromatography due to their physiochemical properties. The large size of IgMs presents a purification challenge. A new AEX resin has been developed to overcome the multiple issues faced when purifying large biomolecules.