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If you'd like to learn more about this topic, the following article may be of interest to you.
Low Glucose Cultivation of CHO cells
by Advanced Control System, BR1000
Low glucose cell cultivation (eg. ～1 g/L) for biopharmaceutical production requires frequent sampling and
frequent glucose supplementation. The excessive sampling requirement not only reduces the volume of the
culture medium, but also is an unnecessary burden on the operators when performed manually. Here, we
demonstrated that the Advanced Control Bioreactor System BR1000 can effectively automate mammalian
cell cultivation under low glucose conditions.
The BR1000 system controls glucose concentration using model predictive control software architecture. The
software algorithms, predicts glucose consumption by leveraging the combined data from direct glucose inline measurements obtained by near-infrared (NIR) spectroscopy, and from viable cell density data obtained
by bio-capacitance measurements.
The BR1000 routinely updates the calibration model using the spectrum data from cultivation to improve the
accuracy of glucose monitoring and glucose concentration control. This application note shows that the
BR1000 can effectively operate under low glucose cultivation (1 g/L) conditions with CHO cells.
Yokogawa Corporation of America will be displaying the BR1000 Advanced Control Bioreactor System at the BioProcess International Exhibition from Sept.28-30 held in the Boston Convention & Exhibition Center. Please stop by booth #1242 to see the BR1000 unit in operation.
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Sponsored by:
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