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Enhancing the Sensitivity
Of Top-Down and Bottom-up
Proteomic Applications Using
The New Halo 1.5 MM ID Column
Decreasing the internal diameter of a column can increase detector response, lower solvent consumption, and bring multiple benefits to the overall analysis workflow. This has fueled the development of capillary columns which provide very high performance, but require specialized instruments in order to realize that performance. Here we present the HALO® 1.5 mm ID column, which shows enhanced sensitivity and solvent savings on a conventional UHPLC system. The only modification required to realize this performance is the reduction of extra column volume, which can easily be done by the consumer.
Download Technical Report |
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Impact of Column ID on Sensitivity for Peptides and Proteins
Using HALO 160 Å ES-C18
Sensitivity in UV applications can be increased with simple changes in column dimensions. It is known that by switching to smaller ID columns, the response from the UV detector increases. The comparisons above shows the impact of column dimensions on sensitivity for a mix of peptides and small proteins. By reducing the column ID there is a significant increase in sensitivity. Without a sufficiently low dispersion UHPLC, the 1.0mm suffers from broad peaks, causing reduced resolution.
Download Application Note |
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Sponsored by:
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