In our October issue:
COLUMNS
Column Watch
Highlights from the HPLC 2019 Symposium
David S. Bell
This "Column Watch" installment reports on highlights from the 48th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2019), held June 16 to 20 in Milan, Italy.
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LC Troubleshooting
Minimizing Fluctuating Peptide Retention in 2D-LC: How to Address a Moving Target
Patrik Petersson
In this installment, tips, tricks, and suggestions are provided for best practices in reversed-phase separations, in both 1D and 2D LC, with gradient elution for minimum variations in retention time.
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Perspectives in Modern HPLC
Ultraviolet Detectors: Perspectives, Principles, and Practices
Michael W. Dong and Jedrzej Wysocki
The authors provide a technical overview of the design and operating principles of variable wavelength and photodiode array detectors, and include historical perspectives and common practices in operation and maintenance.
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Non-targeted Screening for Environmental Water Quality Monitoring
Thursday, November 14, 2019 at 11am EST | 8am PST | 4pm GMT | 5pm CET
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The Essentials
What a GC–MS Tune Report Can Tell You
An essential tool for troubleshooting and tuning GC–MS instruments is the tune report; this article explains the meaning and uses of this report when combined with a tuning compound.
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RPLC Method Development Strategies Using Stationary Phase and Mobile Phase Screening, and Some Helpful Tools and Tips
Wednesday, November 13, 2019 at 1pm EST| 12pm CST| 10am PST
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Feature Article
Data Integrity Focus, Part IX: Understanding Data Integrity Metrics for Chromatography
R.D. McDowall
Here, we review the regulatory expectations of metrics for monitoring data integrity and discuss their practical implementation in a regulated chromatography laboratory.
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Identifying and Quantifying Aspartate and Iso-Aspartate Isomers and Other Difficult PTMs in Proteins
Thursday, November 7, 2019 at 11am EST | 8am PST | 4pm GMT | 5pm CET
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Peer-Reviewed Article
Sampling: The Ghost in Front of the Laboratory Door
Veronika R. Meyer
How does one meet the most demanding part of an analysis—sampling? A typical example is soil, which presents a twofold problem for the analyst: first, the selection of the sites where the samples are taken, and second, the reduction of a sample (for example, 1 kg) to the analysis aliquot size (for example, 10 μL). This paper describes the details of sampling issues.
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How to Approach Preparative LC for Compound Purification in the Research and Development Laboratory
Thursday, November 7, 2019 at 11am EST | 8am PST | 4pm GMT | 5pm CET
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Applications of Field Flow Fractionation
Tuesday, October 29, 2019 at 11am EDT | 8am PDT| 3pm GMT| 4pm CET
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